This role will focus on the Discovery of Antibody based biologics and T/NK cell products as well as assist with planning and execution of experiments, in support of developing analytical methods for releasing and characterizing T cell therapy products. Candidates with strong expertise in phage display library construction and antibody engineering will be given preference.
location: Arcadia, California
job type: Permanent
salary: $100,000 - 130,000 per year
work hours: 9 to 5
education: Masters
responsibilities:
- Use expertise in molecular biology to generate Chimeric Antigen Receptor (CAR) or antibody constructs.
- Engineer lentiviral vectors with insertion or deletion of restriction sites, epitope tags or other genetic elements.
- Generate viral vectors expressing scFv or CAR variants, site directed mutants, and fusion proteins using fusion PCR and Gibson or other fragment assembly methods.
- Design and clone shRNA, gRNA for desired targets in CRISPR/Cas9 vectors.
- Design strategies to knock-in CAR in T cells
- Perform antibody phage display screens and validation of clones by ELISA and flow cytometry.
- Express/Purify antibodies and recombinant proteins in bacteria or mammalian cells.
- Develop biochemical, cell based and immunoassays to evaluate antibody binding affinities
- Optimize qPCR/ddPCR assays to characterize viral and CAR-T products.
- Construct phage display libraries
- Perform restriction analysis, ligation, transformation, colony PCR screens and gel electrophoresis.
- Develop generate stable cell lines with overexpression or knock out (CRISPR shRNA).
- Prepare plasmids in small scale and large scale, maintain plasmid and bacterial glycerol stocks.
- Maintain cell lines, prepare cell banks (adherent and suspension cell lines) as required.
- Perform cytokine ELISAs and Western blotting.
- Perform cell-based (proliferation, cytotoxicity, transcription factor reporter) assays in multi well formats, analyze and clearly document data.
- Perform experiments with minimal supervision, think critically and demonstrate troubleshooting and problem-solving skills.
- Self-motivated, pays attention to details, and willing to accept responsibilities outside of the initial job description
- Develop precise SOPs complying with GLP environment and maintain excellent records that allow systematic tracking and protocol transfer.
- Basic proficiency in various software (Excel, PowerPoint, Graph Pad Prism, Photoshop and Plasmid design/analysis software)
qualifications:
- MS Degree preferably with at least 5+ years of laboratory experience or PhD with 2+ years of laboratory experience in the field of Immunology, cell biology, virology, or molecular biology.
- Experience in phage display library construction, antibody engineering, humanization or affinity maturation of antibodies are preferred.
- Strong technical skills in advanced molecular cloning and fragment assembly techniques are required
- Strong experience in cell based and biochemical assays are required - must be able to perform assays in 96 and 384 well formats with different formats without confusion.
- Experience in working with multiple cell lines (both suspension and adherent) and setting up cell-based assays.
- General lab experience in operating and maintaining basic lab equipment (centrifuge, microscope, PCR machines, nanodrop, DNA and protein electrophoresis systems).
- Hands on experience with sterile microbiology and molecular biology techniques not limited to cloning, mutagenesis, restriction digestion, ligation, transformation, colony PCR, culturing bacterial cells, plasmid preparations.
- Critical to have the ability to maintain accurate records and back track samples prepared without confusion.
- Prior experience in optimizing and trouble-shooting qPCR and ddPCR
- Communicate ideas and results clearly and effectively (both verbally and in writing) to the team members.
- Must feel comfortable in a fast-paced small company environment with minimal direction and able to adjust workload based upon changing priorities
- Ability to adapt to startup environment and make the best use of the existing resources to helping the team to move towards perfection
- Familiarity with routine lab operations and safety procedures.
skills: Biology, Chemistry, GLP (Good Laboratory Practice), ICH Regulations, GMP (Good Manufacturing Practice)
Equal Opportunity Employer: Race, Color, Religion, Sex, Sexual Orientation, Gender Identity, National Origin, Age, Genetic Information, Disability, Protected Veteran Status, or any other legally protected group status.
EOEDisclaime For certain assignments, Covid-19 vaccination and/or testing may be required by Randstad's client or applicable federal mandate, subject to approved medical or religious accommodations. Carefully review the job posting for details on vaccine/testing requirements or ask your Randstad representative for more information.
