Molecular Biologist

  • location: Andover, MA
  • type: Contract
  • salary: $30.19 - $35.52 per hour
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job description

Molecular Biologist

job summary:
Cell Line and Cell Culture Technical Specialist

The candidate will be working on employing molecular biology techniques to genetically engineer mammalian cell lines by over-expressing, knocking down or knocking out various gene targets.

  • The candidate will be evaluating the engineered cells for the desired genotype and phenotype using shake flask and small scale bioreactor experiments.
  • The candidate will have thorough knowledge of molecular and mammalian cell biology.
 
location: Andover, Massachusetts
job type: Contract
salary: $30.19 - 35.52 per hour
work hours: 9 to 5
education: Bachelors
 
responsibilities:
  • Construction and verification of complex DNA vectors
  • Generation and characterization of genetically engineered cell lines
  • Knock-down or knock-out of gene targets using miRNA, siRNA, and CRISPR / Cas9 technology
  • Assess gene expression using RT qPCR
  • Perform western blot analysis to query the protein expression of various target genes.
  • When required, the candidate will also be working closely with the scientists to perform cell maintenance and conduct cell culture experiments in shake-flasks, bioreactors, miniature bioreactors (ambr15) etc.
 
qualifications:
  • BS/BA, MS/MA or PhD/DSc in Chemical Engineering, Biotechnology, Biochemical Engineering, Microbiology, Cell Biology, Biochemistry, Biology or equivalent fields
  • 0-2 years industrial experience preferred.
  • Course work and/or past experience in the cellular metabolism is preferred.
 
skills: Cell Biology, Cell Cultures
Equal Opportunity Employer: Race, Color, Religion, Sex, Sexual Orientation, Gender Identity, National Origin, Age, Genetic Information, Disability, Protected Veteran Status, or any other legally protected group status.

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